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pCAIP-hXRCC1pd-eGFP
(Plasmid #206033)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206033 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES-puro
  • Backbone manufacturer
    Clonetec
  • Backbone size w/o insert (bp) 4476
  • Total vector size (bp) 8880
  • Modifications to backbone
    original CMV promoter was exchanged with a CAG promoter
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hXRCC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4041
  • Mutation
    S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A
  • Promoter CAG
  • Tag / Fusion Protein
    • eGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoNI (not destroyed)
  • 5′ sequencing primer pCAGGS-5
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    the sequence containing the mutated PAR-acceptor sites and mentioned restriction sites was ordered with Twist Bioscience
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAIP-hXRCC1pd-eGFP was a gift from Primo Schaer (Addgene plasmid # 206033 ; http://n2t.net/addgene:206033 ; RRID:Addgene_206033)

  • For your References section:

    Covalent PARylation of DNA base excision repair proteins regulates DNA demethylation. Schwarz SD, Xu J, Gunasekera K, Schurmann D, Vagbo CB, Ferrari E, Slupphaug G, Hottiger MO, Schar P, Steinacher R. Nat Commun. 2024 Jan 2;15(1):184. doi: 10.1038/s41467-023-44209-8. 10.1038/s41467-023-44209-8 PubMed 38167803
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