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Addgene

pBSII-FRT-MCS
(Plasmid #205988)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205988 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescriptII SK+
  • Modifications to backbone
    Replaced original multiple cloning site with a variation, containing FRT and FRT-F3 sites for Flp recombinase
  • Vector type
    PCR vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBSII-FRT-MCS was a gift from Sharon Torigoe (Addgene plasmid # 205988 ; http://n2t.net/addgene:205988 ; RRID:Addgene_205988)

  • For your References section:

    A suboptimal OCT4-SOX2 binding site facilitates the naive-state specific function of a Klf4 enhancer. Waite JB, Boytz R, Traeger AR, Lind TM, Lumbao-Conradson K, Torigoe SE. PLoS One. 2024 Sep 30;19(9):e0311120. doi: 10.1371/journal.pone.0311120. eCollection 2024. 10.1371/journal.pone.0311120 PubMed 39348365
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