FSW_iGluSnFR3.857.GPI
(Plasmid
#205986)
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PurposeExpresses hSyn1-driven iGluSnFR3
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205986 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneFUGW
- Backbone size w/o insert (bp) 7944
- Total vector size (bp) 10202
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Modifications to backboneUbiC promoter replaced with hSyn1 promoter
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameiGluSnFR2.857.GPI
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SpeciesSynthetic
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Insert Size (bp)1791
- Promoter hSyn1
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer gggaggggaaacaggatg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byRecloned from Addgene # 178331
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FSW_iGluSnFR3.857.GPI was a gift from Ronald Hart (Addgene plasmid # 205986 ; http://n2t.net/addgene:205986 ; RRID:Addgene_205986)
Map uploaded by the depositor.
