pCX0393
(Plasmid #205984)

• Purpose: Ga0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacer
• Depositing Lab: Feng Zhang
• Publication: Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910. doi: 10.1126/science.adi1910. Epub 2023 Nov 23.

Backbone

• Vector backbone: pET45b(+)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Ga0116245_10002768 candidate type VII proteins
• Species: Active sludge microbial communities of municipal wastewater-treating anaerobic digesters from various locations
• Promoter: T7 (proteins), pJ23119 (crRNA)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning

Gene/Insert 2

• Gene/Insert name: Cas14
• Tag / Fusion Protein:
  • TwinStrep-tag (C terminal on insert)

Gene/Insert 3

• Gene/Insert name: Cas5
• Tag / Fusion Protein:
  • 14xHis (N terminal on insert)

Gene/Insert 4

• Gene/Insert name: truncated CRISPR array

Resource Information

• Supplemental Documents:
  • pCX0393 Ga0116245_10002768 T7LacO-Cas6 Cas15-TwinStrep Cas7 His14-Cas5pJ23119-truncated DR-truncated Fn spacer.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry