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Addgene

pHS1950
(Plasmid #205983)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205983 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET45b(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Ga0116245_10002768 candidate type VII proteins
  • Species
    Active sludge microbial communities of municipal wastewater-treating anaerobic digesters from various locations
  • Promoter T7 (proteins), pJ23119 (crRNA)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning

Gene/Insert 2

  • Gene/Insert name
    Cas14
  • Tag / Fusion Protein
    • TwinStrep-tag (N terminal on insert)

Gene/Insert 3

  • Gene/Insert name
    Cas5
  • Tag / Fusion Protein
    • 14xHis

Gene/Insert 4

  • Gene/Insert name
    CRISPR array

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

R197S frameshift mutation in Cas7 ORF will not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHS1950 was a gift from Feng Zhang (Addgene plasmid # 205983 ; http://n2t.net/addgene:205983 ; RRID:Addgene_205983)

  • For your References section:

    Uncovering the functional diversity of rare CRISPR-Cas systems with deep terascale clustering. Altae-Tran H, Kannan S, Suberski AJ, Mears KS, Demircioglu FE, Moeller L, Kocalar S, Oshiro R, Makarova KS, Macrae RK, Koonin EV, Zhang F. Science. 2023 Nov 24;382(6673):eadi1910. doi: 10.1126/science.adi1910. Epub 2023 Nov 23. 10.1126/science.adi1910 PubMed 37995242
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