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CL20_mEGFP_AFF1_RAD51B
(Plasmid #205769)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205769 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CL20
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AFF1_RAD51B
  • Species
    H. sapiens (human)
  • Entrez Gene
    AFF1 (a.k.a. AF4, FEL, MLLT2, PBM1)
  • Entrez Gene
    RAD51B (a.k.a. R51H2, RAD51L1, REC2)
  • Tag / Fusion Protein
    • mEGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We find a 52 bp deletion in the ori.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CL20_mEGFP_AFF1_RAD51B was a gift from Richard Kriwacki (Addgene plasmid # 205769 ; http://n2t.net/addgene:205769 ; RRID:Addgene_205769)

  • For your References section:

    Defining the condensate landscape of fusion oncoproteins. Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG, Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni N, Kriwacki RW. Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2. 10.1038/s41467-023-41655-2 PubMed 37770423
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