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Addgene

pAAV-CAG-JNKKTRrsGreenF-E-2A-ERKKTRrsFastLime-IRES-PKAKTRClover-2A-P38KTRDronpa
(Plasmid #205766)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205766 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-CAG
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    JNK KTR rsGreenF-E-2A-ERK KTR rsFastLime-IRES-PKA KTR Clover-2A-P38 KTR Dronpa
  • Species
    Synthetic
  • Insert Size (bp)
    4702
  • Mutation
    wt

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.08.22.504781v1.full for bioRxiv preprint.

Please note: This plasmid contains a 22bp deletion in the right ITR sequence. It is not known how this deletion will impact the function of the ITR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CAG-JNKKTRrsGreenF-E-2A-ERKKTRrsFastLime-IRES-PKAKTRClover-2A-P38KTRDronpa was a gift from Edward Boyden (Addgene plasmid # 205766 ; http://n2t.net/addgene:205766 ; RRID:Addgene_205766)

  • For your References section:

    Temporally multiplexed imaging of dynamic signaling networks in living cells. Qian Y, Celiker OT, Wang Z, Guner-Ataman B, Boyden ES. Cell. 2023 Dec 7;186(25):5656-5672.e21. doi: 10.1016/j.cell.2023.11.010. Epub 2023 Nov 28. 10.1016/j.cell.2023.11.010 PubMed 38029746
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