pLL3.7m-YFP-SLBP(18-126)-IRES-H1-Skylan62A
(Plasmid
#205760)
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PurposeSingle-color FUCCI4 sensor for S/G2 cell cycle transition and G2/M transition
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 205760 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePLL3.7m
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYFP-SLBP(18-126)-IRES-H1-Skylan62A
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SpeciesSynthetic
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Insert Size (bp)2965
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Mutationwt
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.08.22.504781v1.full for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLL3.7m-YFP-SLBP(18-126)-IRES-H1-Skylan62A was a gift from Edward Boyden (Addgene plasmid # 205760 ; http://n2t.net/addgene:205760 ; RRID:Addgene_205760) -
For your References section:
Temporally multiplexed imaging of dynamic signaling networks in living cells. Qian Y, Celiker OT, Wang Z, Guner-Ataman B, Boyden ES. Cell. 2023 Dec 7;186(25):5656-5672.e21. doi: 10.1016/j.cell.2023.11.010. Epub 2023 Nov 28. 10.1016/j.cell.2023.11.010 PubMed 38029746