pWZL Neo Myr Flag PIK3R5
(Plasmid #20576)

• Depositing Labs: William Hahn, Jean Zhao
• Publication: Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Backbone

• Vector backbone: pWZL-Neo-Myr-Flag-DEST
• Backbone manufacturer: William Hahn Lab (available at Addgene, #15300)
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3 cells to prevent recombination.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PIK3R5
• Alt name: phosphoinositide-3-kinase, regulatory subunit 5, p101
• Species: H. sapiens (human)
• GenBank ID: NM_014308
• Entrez Gene: PIK3R5 (a.k.a. F730038I15Rik, FOAP-2, P101-PI3K, p101)
• Tags / Fusion Proteins:
  • Myr (N terminal on backbone)
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: pWZL-Fwd (GAA CCT CCT CGT TCG ACC)
• 3′ sequencing primer: pWZL-Rev (TTC CGG GCC CTC ACA TTG)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: 4-G7
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.

This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021

There is a mutation in the sequence causing S3C and E58G substitution in the aa sequence.