pC081: pAAV.CMV.mCherry
(Plasmid #205744)

• Purpose: AAV vector for expressing CMV-driven mCherry reporter gene.
• Depositing Lab: Guei-Sheung Liu
• Publication: Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121(45):e2408345121. doi: 10.1073/pnas.2408345121. Epub 2024 Oct 30.

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Backbone

• Vector backbone: pAAV.CMV.MCS
• Modifications to backbone: Insert mCherry
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry
• Species: Synthetic; Discosoma sp.
• Insert Size (bp): 711
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CGCAAATGGGCGGTAGGCGTG

Resource Information

• Supplemental Documents:
  • paav-mcherry-rl.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry