pGATE-1
(Plasmid
#205743)
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Purpose(Empty Backbone) Cloning of PCR products by overlap extension into a gateway entry vector
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205743 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR207
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Backbone manufacturerInvitrogen
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Vector typeCloning
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Gentamicin, 25 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGATE-1 was a gift from Viktor Kirik (Addgene plasmid # 205743 ; http://n2t.net/addgene:205743 ; RRID:Addgene_205743) -
For your References section:
Overlap extension cloning of PCR products into a Gateway-compatible plasmid vector. Zare-Mehrjerdi O, Trader G, Kirik V. Biotechniques. 2023 Jul 9. doi: 10.2144/btn-2023-0001. 10.2144/btn-2023-0001 PubMed 37424091
Map uploaded by the depositor.
