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Addgene

LCAPGW
(Plasmid #205642)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205642 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX-Puro
  • Backbone manufacturer
    Clontech
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miR-AB
  • Species
    Synthetic
  • Promoter hCMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site ApaI (unknown if destroyed)
  • 3′ sequencing primer PGK Reverse: GCCAGAGGCCACTTGTGTAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LCAPGW was a gift from Dapeng Wang (Addgene plasmid # 205642 ; http://n2t.net/addgene:205642 ; RRID:Addgene_205642)

  • For your References section:

    miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded multiplex RNAi at a single-cell level. Wang D, Xiu J, Zhao J, Luo J. EMBO Rep. 2022 Apr 5;23(4):e53691. doi: 10.15252/embr.202153691. Epub 2022 Feb 24. 10.15252/embr.202153691 PubMed 35201651
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