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pcDNA3.1-BCR-ABL-p190-FLAG
(Plasmid #205620)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205620 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BCR-ABL oncogene, p190 isoform b3a2
  • Species
    H. sapiens (human)
  • Mutation
    T117M- Please see depositor comments
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note depositor confirms the T117M compared to tyrosine-protein kinase ABL1 isoform a [Homo sapiens] NP_005148.2 does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-BCR-ABL-p190-FLAG was a gift from Pavel Krejčí (Addgene plasmid # 205620 ; http://n2t.net/addgene:205620 ; RRID:Addgene_205620)

  • For your References section:

    Elucidation of protein interactions necessary for the maintenance of the BCR-ABL signaling complex. Gregor T, Bosakova MK, Nita A, Abraham SP, Fafilek B, Cernohorsky NH, Rynes J, Foldynova-Trantirkova S, Zackova D, Mayer J, Trantirek L, Krejci P. Cell Mol Life Sci. 2020 Oct;77(19):3885-3903. doi: 10.1007/s00018-019-03397-7. Epub 2019 Dec 9. 10.1007/s00018-019-03397-7 PubMed 31820037
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