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pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP
(Plasmid #205604)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205604 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA5/FRT/TO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5137
  • Total vector size (bp) 7166
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    γ-tubulinΔGTP-RNAi-resistant-GFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2088
  • Mutation
    G2228A, C2231T, C2237T, G2240A, N229A
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP was a gift from Tarun Kapoor (Addgene plasmid # 205604 ; http://n2t.net/addgene:205604 ; RRID:Addgene_205604)

  • For your References section:

    A nucleotide binding-independent role for gamma-tubulin in microtubule capping and cell division. Berman AY, Wieczorek M, Aher A, Olinares PDB, Chait BT, Kapoor TM. J Cell Biol. 2023 Mar 6;222(3):e202204102. doi: 10.1083/jcb.202204102. Epub 2023 Jan 25. 10.1083/jcb.202204102 PubMed 36695784
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