pcDNA3.1_ADAR2DD_E488Q_Ago2
(Plasmid
#205599)
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Purposeexpresses human ADAR2 deaminase domain (E488Q hyperactive mutant) fused with human Ago2 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205599 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehAgo2
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Alt nameEIF2C2
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SpeciesH. sapiens (human)
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Entrez GeneAGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)
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Tag
/ Fusion Protein
- ADAR2DD_E488Q (hyperactive mutant) (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.08.10.503472v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1_ADAR2DD_E488Q_Ago2 was a gift from Marc Friedlander (Addgene plasmid # 205599 ; http://n2t.net/addgene:205599 ; RRID:Addgene_205599) -
For your References section:
Detection of transcriptome-wide microRNA-target interactions in single cells with agoTRIBE. Sekar V, Marmol-Sanchez E, Kalogeropoulos P, Stanicek L, Sagredo EA, Widmark A, Doukoumopoulos E, Bonath F, Biryukova I, Friedlander MR. Nat Biotechnol. 2023 Sep 21. doi: 10.1038/s41587-023-01951-0. 10.1038/s41587-023-01951-0 PubMed 37735263
