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Addgene

pGEL682
(Plasmid #205591)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205591 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEL686
  • Backbone size (bp) 17963
  • Modifications to backbone
    ZmUbi derived zCas9(H840) M-MLV, terminated by AtHsp. sgRNA driven by OsU6-26 promoter
  • Vector type
    Plant Expression, CRISPR, Synthetic Biology
  • Promoter ZmUbi
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please Note: This plasmid contains a Q1680P mutation in the zCas9(H840) insert and an IS4-like element inserted downstream of KanR. These are not expected to impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEL682 was a gift from Yong Zhang (Addgene plasmid # 205591 ; http://n2t.net/addgene:205591 ; RRID:Addgene_205591)

  • For your References section:

    An improved plant prime editor for efficient generation of multiple-nucleotide variations and structural variations in rice. Zhong Z, Fan T, He Y, Liu S, Zheng X, Xu Y, Ren J, Yuan H, Xu Z, Zhang Y. Plant Commun. 2024 May 14:100976. doi: 10.1016/j.xplc.2024.100976. 10.1016/j.xplc.2024.100976 PubMed 38751122
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