pAXY0016
(Plasmid
#205585)
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Purposep3flag_HygR(1-89)-NpuDnaE(N)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205585 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGCS-N3(3xFLAG)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHygR-NpuDnaE(N)_homo-codon-OP
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SpeciesSynthetic
- Promoter CMV IE94
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAXY0016 was a gift from Xiaohan Yang (Addgene plasmid # 205585 ; http://n2t.net/addgene:205585 ; RRID:Addgene_205585) -
For your References section:
Split selectable marker systems utilizing inteins facilitate gene stacking in plants. Yuan G, Lu H, De K, Hassan MM, Liu Y, Islam MT, Muchero W, Tuskan GA, Yang X. Commun Biol. 2023 May 26;6(1):567. doi: 10.1038/s42003-023-04950-8. 10.1038/s42003-023-04950-8 PubMed 37237044
Map uploaded by the depositor.
