pAXY0012
(Plasmid
#205581)
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PurposeHYG(1-52)-NpuDnaE(N)_GFPuv
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 205581 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAXY0002
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Vector typePlant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNpuDnaE(N)
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SpeciesSynthetic
- Promoter 35S
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAXY0012 was a gift from Xiaohan Yang (Addgene plasmid # 205581 ; http://n2t.net/addgene:205581 ; RRID:Addgene_205581) -
For your References section:
Split selectable marker systems utilizing inteins facilitate gene stacking in plants. Yuan G, Lu H, De K, Hassan MM, Liu Y, Islam MT, Muchero W, Tuskan GA, Yang X. Commun Biol. 2023 May 26;6(1):567. doi: 10.1038/s42003-023-04950-8. 10.1038/s42003-023-04950-8 PubMed 37237044