Skip to main content
Addgene

WIW_Scaffold2
(Plasmid #205574)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205574 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMES4
  • Backbone manufacturer
    NovoPro Bioscience
  • Backbone size w/o insert (bp) 4365
  • Total vector size (bp) 4806
  • Modifications to backbone
    N-terminal pelB signal peptide for periplasmic expression
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    WIW_Scaffold2
  • Species
    Synthetic
  • Insert Size (bp)
    441
  • Tag / Fusion Protein
    • 6X His (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    WIW_Scaffold2 was a gift from David Eisenberg (Addgene plasmid # 205574 ; http://n2t.net/addgene:205574 ; RRID:Addgene_205574)

  • For your References section:

    Structure-based design of nanobodies that inhibit seeding of Alzheimer's patient-extracted tau fibrils. Abskharon R, Pan H, Sawaya MR, Seidler PM, Olivares EJ, Chen Y, Murray KA, Zhang J, Lantz C, Bentzel M, Boyer DR, Cascio D, Nguyen BA, Hou K, Cheng X, Pardon E, Williams CK, Nana AL, Vinters HV, Spina S, Grinberg LT, Seeley WW, Steyaert J, Glabe CG, Ogorzalek Loo RR, Loo JA, Eisenberg DS. Proc Natl Acad Sci U S A. 2023 Oct 10;120(41):e2300258120. doi: 10.1073/pnas.2300258120. Epub 2023 Oct 6. 10.1073/pnas.2300258120 PubMed 37801475
Related items: