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pcDNA3.1(+)-Golgi-RasAR
(Plasmid #205570)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205570 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5363
  • Total vector size (bp) 7526
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Golgi-RasAR
  • Alt name
    Cerulean3-RBD(Raf1 51-131)-pseudoligand-YPet-Giantin
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2163
  • Entrez Gene
    RAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)
  • Entrez Gene
    GOLGB1 (a.k.a. GCP, GCP372, GOLIM1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Golgin B1 (Giantin) residues 3131-3259 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)-Golgi-RasAR was a gift from Jin Zhang (Addgene plasmid # 205570 ; http://n2t.net/addgene:205570 ; RRID:Addgene_205570)

  • For your References section:

    Fluorescent Biosensor for Measuring Ras Activity in Living Cells. Weeks R, Zhou X, Yuan TL, Zhang J. J Am Chem Soc. 2022 Sep 28;144(38):17432-17440. doi: 10.1021/jacs.2c05203. Epub 2022 Sep 19. 10.1021/jacs.2c05203 PubMed 36122391
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