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pFH2.155_LV-Puro-T2A-mTagBFP2
(Plasmid #205553)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205553 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-EF1-MCS
  • Backbone manufacturer
    System Biosciences
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin ; mTagBFP2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Puro-T2A-mTagBFP2
  • Species
    Synthetic
  • Mutation
    WT
  • Promoter EF1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFH2.155_LV-Puro-T2A-mTagBFP2 was a gift from Michael Elowitz (Addgene plasmid # 205553 ; http://n2t.net/addgene:205553 ; RRID:Addgene_205553)

  • For your References section:

    Engineering RNA export for measurement and manipulation of living cells. Horns F, Martinez JA, Fan C, Haque M, Linton JM, Tobin V, Santat L, Maggiolo AO, Bjorkman PJ, Lois C, Elowitz MB. Cell. 2023 Jul 10:S0092-8674(23)00689-X. doi: 10.1016/j.cell.2023.06.013. 10.1016/j.cell.2023.06.013 PubMed 37437570
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