pFH2.98_PB-EPN1-MCP_Myc-T2A-eGFP-PGK-Blast
(Plasmid
#205543)
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PurposeExpress EPN1-MCP-Myc-T2A-eGFP and blasticidin resistance marker
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 205543 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneePB2
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Vector typeMammalian Expression ; Piggybac
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Selectable markersBlasticidin ; eGFP
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEPN1-MCP-Myc
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SpeciesSynthetic
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MutationWT
- Promoter EF1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFH2.98_PB-EPN1-MCP_Myc-T2A-eGFP-PGK-Blast was a gift from Michael Elowitz (Addgene plasmid # 205543 ; http://n2t.net/addgene:205543 ; RRID:Addgene_205543) -
For your References section:
Engineering RNA export for measurement and manipulation of living cells. Horns F, Martinez JA, Fan C, Haque M, Linton JM, Tobin V, Santat L, Maggiolo AO, Bjorkman PJ, Lois C, Elowitz MB. Cell. 2023 Jul 10:S0092-8674(23)00689-X. doi: 10.1016/j.cell.2023.06.013. 10.1016/j.cell.2023.06.013 PubMed 37437570