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pFH2.97_PB-EPN1-MCP-T2A-eGFP-PGK-Blast
(Plasmid #205542)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205542 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ePB2
  • Vector type
    Mammalian Expression ; Piggybac
  • Selectable markers
    Blasticidin ; eGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EPN1-MCP
  • Species
    Synthetic
  • Mutation
    WT
  • Promoter EF1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFH2.97_PB-EPN1-MCP-T2A-eGFP-PGK-Blast was a gift from Michael Elowitz (Addgene plasmid # 205542 ; http://n2t.net/addgene:205542 ; RRID:Addgene_205542)

  • For your References section:

    Engineering RNA export for measurement and manipulation of living cells. Horns F, Martinez JA, Fan C, Haque M, Linton JM, Tobin V, Santat L, Maggiolo AO, Bjorkman PJ, Lois C, Elowitz MB. Cell. 2023 Jul 10:S0092-8674(23)00689-X. doi: 10.1016/j.cell.2023.06.013. 10.1016/j.cell.2023.06.013 PubMed 37437570
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