pcDNA3-nuc-Nb80
(Plasmid #205519)

• Purpose: Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.
• Depositing Lab: Jin Zhang
• Publication: Kwon et al Nature. 2022 Nov;611(7934):173-179. doi: 10.1038/s41586-022-05343-3. Epub 2022 Oct 26.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5325
• Total vector size (bp): 6459
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: nuc-Nb80
• Alt name: mCherry-Nb37-NLS
• Species: Synthetic
• Insert Size (bp): 1134
• Promoter: CMV
• Tags / Fusion Proteins:
  • mCherry (N terminal on insert)
  • Nuclear localization signal (NLS) (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Nb80 cDNA was provided by Roger Sunahara (UCSD)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry