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pcDNA3-endo-EKAR4
(Plasmid #205513)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205513 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5472
  • Total vector size (bp) 7965
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    endo-EKAR4
  • Alt name
    EKAR4-2xFYVE
  • Species
    Synthetic
  • Insert Size (bp)
    2493
  • Promoter CMV
  • Tags / Fusion Proteins
    • 6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag (N terminal on backbone)
    • 2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-endo-EKAR4 was a gift from Jin Zhang (Addgene plasmid # 205513 ; http://n2t.net/addgene:205513 ; RRID:Addgene_205513)

  • For your References section:

    Non-canonical beta-adrenergic activation of ERK at endosomes. Kwon Y, Mehta S, Clark M, Walters G, Zhong Y, Lee HN, Sunahara RK, Zhang J. Nature. 2022 Nov;611(7934):173-179. doi: 10.1038/s41586-022-05343-3. Epub 2022 Oct 26. 10.1038/s41586-022-05343-3 PubMed 36289326
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