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Addgene

pDRF1-GW ymTq2-d11
(Plasmid #205490)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205490 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDRF1-GW
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ymTq2-d11
  • Alt name
    ymTurquoise2-d11
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Additional publication for this plasmid:
An Improved ATP FRET Sensor For Yeast Shows Heterogeneity During Nutrient Transitions
Dennis Botman, Johan H. van Heerden, and Bas Teusink
ACS Sensors 2020 5 (3), 814-822 DOI: 10.1021/acssensors.9b02475
https://pubmed.ncbi.nlm.nih.gov/32077276/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDRF1-GW ymTq2-d11 was a gift from Bas Teusink (Addgene plasmid # 205490 ; http://n2t.net/addgene:205490 ; RRID:Addgene_205490)
  • For your References section:

    A fast method to distinguish between fermentative and respiratory metabolisms in single yeast cells. Luzia L, Battjes J, Zwering E, Jansen D, Melkonian C, Teusink B. iScience. 2023 Dec 21;27(1):108767. doi: 10.1016/j.isci.2023.108767. eCollection 2024 Jan 19. 10.1016/j.isci.2023.108767 PubMed 38235328