pDRF1-GW ymTq2-d11
(Plasmid
#205490)
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Purposeexpress ymTq2-d11 as donor control for yAT1.03
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 205490 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDRF1-GW
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameymTq2-d11
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Alt nameymTurquoise2-d11
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Additional publication for this plasmid:
An Improved ATP FRET Sensor For Yeast Shows Heterogeneity During Nutrient Transitions
Dennis Botman, Johan H. van Heerden, and Bas Teusink
ACS Sensors 2020 5 (3), 814-822 DOI: 10.1021/acssensors.9b02475
https://pubmed.ncbi.nlm.nih.gov/32077276/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDRF1-GW ymTq2-d11 was a gift from Bas Teusink (Addgene plasmid # 205490 ; http://n2t.net/addgene:205490 ; RRID:Addgene_205490) -
For your References section:
A fast method to distinguish between fermentative and respiratory metabolisms in single yeast cells. Luzia L, Battjes J, Zwering E, Jansen D, Melkonian C, Teusink B. iScience. 2023 Dec 21;27(1):108767. doi: 10.1016/j.isci.2023.108767. eCollection 2024 Jan 19. 10.1016/j.isci.2023.108767 PubMed 38235328