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pWZL Neo Myr Flag NEK3
(Plasmid #20547)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20547 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWZL-Neo-Myr-Flag-DEST
  • Backbone manufacturer
    William Hahn Lab (available at Addgene, #15300)
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3 cells to prevent recombination.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NEK3
  • Alt name
    NIMA (never in mitosis gene a)-related kinase 3
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_002498
  • Entrez Gene
    NEK3 (a.k.a. HSPK36)
  • Tags / Fusion Proteins
    • Myr (N terminal on backbone)
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer pWZL-Fwd (GAA CCT CCT CGT TCG ACC)
  • 3′ sequencing primer pWZL-Rev (TTC CGG GCC CTC ACA TTG)
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    4-C8

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.

This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWZL Neo Myr Flag NEK3 was a gift from William Hahn & Jean Zhao (Addgene plasmid # 20547 ; http://n2t.net/addgene:20547 ; RRID:Addgene_20547)

  • For your References section:

    Integrative Genomic Approaches Identify IKBKE as a Breast Cancer Oncogene (Kinase Library Clones). Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK, Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES, Polyak K, Hahn WC. Cell. 2007 Jun 15. 129(6):1065-1079. 10.1016/j.cell.2007.03.052 PubMed 17574021
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