pGL3-MTS-CCDB-N136-RsDddA-C-UGI-PURO
(Plasmid
#205465)
-
PurposeA backbone containing a RsDddA fragment C for constructing mitochondria-targeted RsDdCBE expression plasmid
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205465 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepGL3
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)ccdB Survival
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameMTS; N136; ccdb; RsDddA-C; UGI
-
SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGL3-MTS-CCDB-N136-RsDddA-C-UGI-PURO was a gift from Bin Shen (Addgene plasmid # 205465 ; http://n2t.net/addgene:205465 ; RRID:Addgene_205465) -
For your References section:
Engineering RsDddA as mitochondrial base editor with wide target compatibility and enhanced activity. Cheng K, Li C, Jin J, Qian X, Guo J, Shen L, Dai Y, Zhang X, Li Z, Guan Y, Zhou F, Tang J, Zhang J, Shen B, Lou X. Mol Ther Nucleic Acids. 2023 Sep 9;34:102028. doi: 10.1016/j.omtn.2023.09.005. eCollection 2023 Dec 12. 10.1016/j.omtn.2023.09.005 PubMed 37744175
