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Addgene

pGL3-MTS-CCDB-N136-RsDddA-B-UGI-PURO
(Plasmid #205464)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205464 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MTS; N136; ccdb; RsDddA-B; UGI
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-MTS-CCDB-N136-RsDddA-B-UGI-PURO was a gift from Bin Shen (Addgene plasmid # 205464 ; http://n2t.net/addgene:205464 ; RRID:Addgene_205464)

  • For your References section:

    Engineering RsDddA as mitochondrial base editor with wide target compatibility and enhanced activity. Cheng K, Li C, Jin J, Qian X, Guo J, Shen L, Dai Y, Zhang X, Li Z, Guan Y, Zhou F, Tang J, Zhang J, Shen B, Lou X. Mol Ther Nucleic Acids. 2023 Sep 9;34:102028. doi: 10.1016/j.omtn.2023.09.005. eCollection 2023 Dec 12. 10.1016/j.omtn.2023.09.005 PubMed 37744175
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