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TRE-UBC-hCD47-T2A-GFP
(Plasmid #205458)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205458 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCW
  • Vector type
    Mammalian Expression
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hCD47
  • Species
    H. sapiens (human)
  • Entrez Gene
    CD47 (a.k.a. IAP, MER6, OA3)
  • Promoter UBC

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TRE-UBC-hCD47-T2A-GFP was a gift from Deepta Bhattacharya (Addgene plasmid # 205458 ; http://n2t.net/addgene:205458 ; RRID:Addgene_205458)

  • For your References section:

    Engineering human pluripotent stem cell lines to evade xenogeneic transplantation barriers. Pizzato HA, Alonso-Guallart P, Woods J, Connelly JP, Fehniger TA, Atkinson JP, Pruett-Miller SM, Monsma FJ Jr, Bhattacharya D. Stem Cell Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 10.1016/j.stemcr.2023.12.003. 10.1016/j.stemcr.2023.12.003 PubMed 38215755
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