TRE-UBC-mCD47-T2A-GFP
(Plasmid #205449)

• Purpose: lentiviral plasmid for expression of mouse CD47
• Depositing Lab: Deepta Bhattacharya
• Publication: Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 10.1016/j.stemcr.2023.12.003.

Backbone

• Vector backbone: pCW
• Vector type: Mammalian Expression
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCD47
• Species: M. musculus (mouse)
• Entrez Gene: Cd47 (a.k.a. 9130415E20Rik, B430305P08Rik, IAP, Itgp)
• Promoter: UBC

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • TRE-UBC-mCD47-T2A-GFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.06.27.546594v1 for bioRxiv preprint.

Plasmid has been found to exist as a dimer. Plasmid dimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.