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pAAV-CMV-FLEXfrt-SaCas9-U6-sgNtsr1(guide_2)
(Plasmid #205417)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205417 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-FLEXfrt-SaCas9-U6-sgRNA (#124845)
  • Backbone size w/o insert (bp) 7725
  • Total vector size (bp) 7745
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ntsr1
  • gRNA/shRNA sequence
    CTTGGTGCGGCTGCGGGACAT
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Ntsr1 (a.k.a. NT-1R, NTR-1, NTR1)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer GACTATCATATGCTTACCGT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CMV-FLEXfrt-SaCas9-U6-sgNtsr1(guide_2) was a gift from Larry Zweifel (Addgene plasmid # 205417 ; http://n2t.net/addgene:205417 ; RRID:Addgene_205417)
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