pYPQ301
(Plasmid
#205415)
-
PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Hs1Cas12a without promoter
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 205415 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepYPQ167
- Total vector size (bp) 7149
-
Vector typePlant Expression, CRISPR ; Gateway compatible Cas12a entry clone
Growth in Bacteria
-
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameHs1Cas12a
-
SpeciesHydrogenovibrio sp. XS5
-
Insert Size (bp)3891
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pYPQ301 was a gift from Yiping Qi (Addgene plasmid # 205415 ; http://n2t.net/addgene:205415 ; RRID:Addgene_205415) -
For your References section:
Hs1Cas12a and Ev1Cas12a confer efficient genome editing in plants. Li G, Zhang Y, Dailey M, Qi Y. Front Genome Ed. 2023 Oct 12;5:1251903. doi: 10.3389/fgeed.2023.1251903. eCollection 2023. 10.3389/fgeed.2023.1251903 PubMed 37901282