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Addgene

pYPQ300
(Plasmid #205414)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205414 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYPQ167
  • Backbone size w/o insert (bp) 3258
  • Total vector size (bp) 7032
  • Vector type
    Plant Expression, CRISPR ; Gateway compatible Cas12a entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ev1Cas12a
  • Species
    Eubacterium ventriosum
  • Insert Size (bp)
    3774
  • Tag / Fusion Protein
    • NLS (N terminal on insert) NLS (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ300 was a gift from Yiping Qi (Addgene plasmid # 205414 ; http://n2t.net/addgene:205414 ; RRID:Addgene_205414)

  • For your References section:

    Hs1Cas12a and Ev1Cas12a confer efficient genome editing in plants. Li G, Zhang Y, Dailey M, Qi Y. Front Genome Ed. 2023 Oct 12;5:1251903. doi: 10.3389/fgeed.2023.1251903. eCollection 2023. 10.3389/fgeed.2023.1251903 PubMed 37901282
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