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Addgene

miABE
(Plasmid #205412)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205412 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    HEpl016
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
  • Species
    Synthetic
  • Mutation
    D61A+E85R+H369R+S387R+S457R
  • Promoter hU6, CAG, CMV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Supplemental Documents

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    miABE was a gift from Yingsi Zhou (Addgene plasmid # 205412 ; http://n2t.net/addgene:205412 ; RRID:Addgene_205412)

  • For your References section:

    Development of miniature base editors using engineered IscB nickase. Han D, Xiao Q, Wang Y, Zhang H, Dong X, Li G, Kong X, Wang S, Song J, Zhang W, Zhou J, Bi L, Yuan Y, Shi L, Zhong N, Yang H, Zhou Y. Nat Methods. 2023 May 25. doi: 10.1038/s41592-023-01898-9. 10.1038/s41592-023-01898-9 PubMed 37231266
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