p2301y-tdtomato-dCas9_DegronDead_TPLN188
(Plasmid
#205409)
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PurposedCas9 guided repressor
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205409 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonep2301y
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedCas9_DegronDead_TPLN188
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SpeciesSynthetic
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Insert Size (bp)10638
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
UBQ1-tdTomato-tUBQ1
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p2301y-tdtomato-dCas9_DegronDead_TPLN188 was a gift from Jennifer Nemhauser (Addgene plasmid # 205409 ; http://n2t.net/addgene:205409 ; RRID:Addgene_205409) -
For your References section:
Building a pipeline to identify and engineer constitutive and repressible promoters. Yang EJY, Nemhauser JL. Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. eCollection 2023. 10.1017/qpb.2023.10 PubMed 37901686
