pICH41295(pro5U)-pAT1G64550(E,A)
(Plasmid
#205363)
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PurposeMoClo lvl0 Promoter + 5'UTR part
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 205363 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepICH41295
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Backbone manufacturerSylvestre Marillonnet Lab
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepAT1G64550(E,A)
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)337
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pICH41295(pro5U)-pAT1G64550(E,A) was a gift from Jennifer Nemhauser (Addgene plasmid # 205363 ; http://n2t.net/addgene:205363 ; RRID:Addgene_205363) -
For your References section:
Building a pipeline to identify and engineer constitutive and repressible promoters. Yang EJY, Nemhauser JL. Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. eCollection 2023. 10.1017/qpb.2023.10 PubMed 37901686