pBad33_ISCpe2_reRNA
(Plasmid
#205335)
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PurposeBacterial expression of ISCpe2 TnpB and its reRNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 205335 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAD
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameISCpe2 TnpB
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SpeciesOther
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBad33_ISCpe2_reRNA was a gift from Haoyi Wang (Addgene plasmid # 205335 ; http://n2t.net/addgene:205335 ; RRID:Addgene_205335) -
For your References section:
Evolutionary mining and functional characterization of TnpB nucleases identify efficient miniature genome editors. Xiang G, Li Y, Sun J, Huo Y, Cao S, Cao Y, Guo Y, Yang L, Cai Y, Zhang YE, Wang H. Nat Biotechnol. 2023 Jun 29. doi: 10.1038/s41587-023-01857-x. 10.1038/s41587-023-01857-x PubMed 37386294