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pX601-AAV-CMV-NLS-ISAam1-NLS-3xHA-bGHpA;U6-BbsI-reRNA
(Plasmid #205316)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205316 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    humanized ISAam1 TnpB
  • Species
    Other
  • Tag / Fusion Protein
    • 3 x HA (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX601-AAV-CMV-NLS-ISAam1-NLS-3xHA-bGHpA;U6-BbsI-reRNA was a gift from Haoyi Wang (Addgene plasmid # 205316 ; http://n2t.net/addgene:205316 ; RRID:Addgene_205316)

  • For your References section:

    Evolutionary mining and functional characterization of TnpB nucleases identify efficient miniature genome editors. Xiang G, Li Y, Sun J, Huo Y, Cao S, Cao Y, Guo Y, Yang L, Cai Y, Zhang YE, Wang H. Nat Biotechnol. 2023 Jun 29. doi: 10.1038/s41587-023-01857-x. 10.1038/s41587-023-01857-x PubMed 37386294
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