CaMKAR
(Plasmid
#205315)
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PurposeFluorescent Reporter for CaMKII Activity
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205315 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5408
- Total vector size (bp) 6632
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCaMKAR
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SpeciesSynthetic
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Insert Size (bp)1223
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-Fwd
- 3′ sequencing primer bGH-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CaMKAR was a gift from Mark Anderson & Elizabeth Luczak (Addgene plasmid # 205315 ; http://n2t.net/addgene:205315 ; RRID:Addgene_205315) -
For your References section:
An improved reporter identifies ruxolitinib as a potent and cardioprotective CaMKII inhibitor. Reyes Gaido OE, Pavlaki N, Granger JM, Mesubi OO, Liu B, Lin BL, Long A, Walker D, Mayourian J, Schole KL, Terrillion CE, Nkashama LJ, Hulsurkar MM, Dorn LE, Ferrero KM, Huganir RL, Muller FU, Wehrens XHT, Liu JO, Luczak ED, Bezzerides VJ, Anderson ME. Sci Transl Med. 2023 Jun 21;15(701):eabq7839. doi: 10.1126/scitranslmed.abq7839. Epub 2023 Jun 21. 10.1126/scitranslmed.abq7839 PubMed 37343080
Map uploaded by the depositor.
