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Addgene

pTol2-elavl3-WHaloCaMP1a-EGFP
(Plasmid #205311)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205311 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTol2
  • Vector type
    Zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WHaloCaMP1a-EGFP
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.07.18.549527 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTol2-elavl3-WHaloCaMP1a-EGFP was a gift from Eric Schreiter (Addgene plasmid # 205311 ; http://n2t.net/addgene:205311 ; RRID:Addgene_205311)
  • For your References section:

    A modular chemigenetic calcium indicator enables in vivo functional imaging with near-infrared light. Farrants H, Shuai Y, Lemon WC, Hernandez CM, Yang S, Patel R, Qiao G, Frei MS, Grimm JB, Hanson TL, Tomaska F, Turner GC, Stringer C, Keller PJ, Beyene AG, Chen Y, Liang Y, Lavis LD, Schreiter ER. bioRxiv. 2023 Jul 19:2023.07.18.549527. doi: 10.1101/2023.07.18.549527. Preprint. 10.1101/2023.07.18.549527 PubMed 37503182