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pInt_attP1_sacB_kanR
(Plasmid #205301)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205301 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKD4
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BW25141
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pKM496 (Addgene #109301), pKD4 (CGSC #7632), and pKM461 (Addgene #108320)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is pInt_attP1_kanR with the sacB gene (sucrose sensitivity), which provides counter selection for clean deletions or marker excision. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint. Depositing lab uses BW25141 strain for growth (https://cgsc.biology.yale.edu/Strain.php?ID=64690)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pInt_attP1_sacB_kanR was a gift from Scott Saunders (Addgene plasmid # 205301 ; http://n2t.net/addgene:205301 ; RRID:Addgene_205301)

  • For your References section:

    ORBIT for E. coli: Kilobase-scale oligonucleotide recombineering at high throughput and high efficiency. Saunders SH, Ahmed AM. bioRxiv 2023 10.1101/2023.06.28.546561
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