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Addgene

pInt_attP1_ampR
(Plasmid #205296)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205296 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKD4
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BW25141
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pKM496 (Addgene #109301), pKD4 (CGSC #7632), and pKD46 (CGSC #7739)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Nonreplicating ORBIT integrating plasmid with WT attP (P1) sequence. Replicates in pir+ strains for cloning. FRT sites can be used to excise most of the backbone. Ampicillin resistant. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint. Depositing lab uses BW25141 strain for growth (https://cgsc.biology.yale.edu/Strain.php?ID=64690)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pInt_attP1_ampR was a gift from Scott Saunders (Addgene plasmid # 205296 ; http://n2t.net/addgene:205296 ; RRID:Addgene_205296)

  • For your References section:

    ORBIT for E. coli: Kilobase-scale oligonucleotide recombineering at high throughput and high efficiency. Saunders SH, Ahmed AM. bioRxiv 2023 10.1101/2023.06.28.546561
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