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Addgene

pHelper_Ec1_V1_ampR
(Plasmid #205292)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205292 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKM461
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CspRecT / Bxb-1
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pORTMAGE-Ec1 (Addgene #138474), pKM461 (Addgene #108320), and pKD46 (CGSC #7739).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Replicating ORBIT V1 helper plasmid w/ inducible oligo recombineering module (m-tol) and bxb-1 integrase (arabinose). SacB is present for counterselection and ampicillin is the resistance marker. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHelper_Ec1_V1_ampR was a gift from Scott Saunders (Addgene plasmid # 205292 ; http://n2t.net/addgene:205292 ; RRID:Addgene_205292)

  • For your References section:

    ORBIT for E. coli: Kilobase-scale oligonucleotide recombineering at high throughput and high efficiency. Saunders SH, Ahmed AM. bioRxiv 2023 10.1101/2023.06.28.546561
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