pAAV_EF1a_DIO_jGCaMP8f_nano_ribo_WPRE
(Plasmid
#205283)
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PurposeGCaMP8f fusion to ribosome nanobody to restrict expression to soma (similar to Chen et al. 2020)
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Depositing Lab
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Publication
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205283 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGCaMP8f
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SpeciesM. musculus (mouse)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV_EF1a_DIO_jGCaMP8f_nano_ribo_WPRE was a gift from Matthias Prigge (Addgene plasmid # 205283 ; http://n2t.net/addgene:205283 ; RRID:Addgene_205283)
