pAAV_Ef1a_DIO_MyrPalm_mScarlet_WT_IRES_mNeon_minBack
(Plasmid
#205282)
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PurposeIn order to efficiently visualize projection and cell somata in light-sheet preparations, a combination of membrane-targeted and diffusible red fluorophores was developed. Additionally an ALFA tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 205282 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemScarlet
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Alt namemRubypAAV_Ef1a_DIO_MyrPalm_mScarlet_WT_IRES_MAPKKNES_mRuby_Alfa
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SpeciesM. musculus (mouse)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV_Ef1a_DIO_MyrPalm_mScarlet_WT_IRES_mNeon_minBack was a gift from Matthias Prigge (Addgene plasmid # 205282 ; http://n2t.net/addgene:205282 ; RRID:Addgene_205282)