pAAV_CbH_SIO_SyGCaMP8s_p2a_mRuby
(Plasmid
#205280)
-
PurposeSynaptic activity can be imaged with Synaptophysin GCaMP, while simultaneously, a red fluorescence structural marker can be employed to provide a visual reference for the cellular architecture.
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 205280 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneAAV
-
Vector typeAAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameGCaMP8s
-
Alt namemRuby
-
Alt nameSynaptophysin
-
SpeciesM. musculus (mouse)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAV_CbH_SIO_SyGCaMP8s_p2a_mRuby was a gift from Matthias Prigge (Addgene plasmid # 205280 ; http://n2t.net/addgene:205280 ; RRID:Addgene_205280)