pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)
(Plasmid
#205278)
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PurposeHuman expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 205278 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepU6
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpuFz1 omegaRNA
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SpeciesSpizellomyces punctatus
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M) was a gift from Feng Zhang (Addgene plasmid # 205278 ; http://n2t.net/addgene:205278 ; RRID:Addgene_205278) -
For your References section:
Fanzor is a eukaryotic programmable RNA-guided endonuclease. Saito M, Xu P, Faure G, Maguire S, Kannan S, Altae-Tran H, Vo S, Desimone A, Macrae RK, Zhang F. Nature. 2023 Jun 28. doi: 10.1038/s41586-023-06356-2. 10.1038/s41586-023-06356-2 PubMed 37380027