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Addgene

pMSSM59: pCMV-HA-NLS-SpuFz1-D300R
(Plasmid #205272)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205272 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpuFz1
  • Species
    Spizellomyces punctatus
  • Mutation
    D300R
  • Tag / Fusion Protein
    • HA, NLS(Makkerh) (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSSM59: pCMV-HA-NLS-SpuFz1-D300R was a gift from Feng Zhang (Addgene plasmid # 205272 ; http://n2t.net/addgene:205272 ; RRID:Addgene_205272)

  • For your References section:

    Fanzor is a eukaryotic programmable RNA-guided endonuclease. Saito M, Xu P, Faure G, Maguire S, Kannan S, Altae-Tran H, Vo S, Desimone A, Macrae RK, Zhang F. Nature. 2023 Jun 28. doi: 10.1038/s41586-023-06356-2. 10.1038/s41586-023-06356-2 PubMed 37380027
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