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Addgene

KRAB-dSpCas9
(Plasmid #205248)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205248 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    IVTpT7
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KRAB-dSpCas9
  • Species
    Synthetic

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert is derived from Addgene plasmid #85449

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    KRAB-dSpCas9 was a gift from Rasmus Bak (Addgene plasmid # 205248 ; http://n2t.net/addgene:205248 ; RRID:Addgene_205248)

  • For your References section:

    Targeted regulation of transcription in primary cells using CRISPRa and CRISPRi. Jensen TI, Mikkelsen NS, Gao Z, Fosselteder J, Pabst G, Axelgaard E, Laustsen A, Konig S, Reinisch A, Bak RO. Genome Res. 2021 Nov;31(11):2120-2130. doi: 10.1101/gr.275607.121. Epub 2021 Aug 18. 10.1101/gr.275607.121 PubMed 34407984
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